Tick-Borne Encephalitis Virus Cleavage-Deficient Mutant of the Flavivirus Resuscitating Mutations in a Furin
نویسندگان
چکیده
منابع مشابه
Changing the protease specificity for activation of a flavivirus, tick-borne encephalitis virus.
The infectivity of flavivirus particles depends on a maturation process that is triggered by the proteolytic cleavage of the precursor of the M protein (prM). This activation cleavage is naturally performed by ubiquitous cellular proteases of the furin family, which typically recognize the multibasic sequence motif R-X-R/K-R. Previously, we demonstrated that a tick-borne encephalitis virus (TBE...
متن کاملSpontaneous mutations restore the viability of tick-borne encephalitis virus mutants with large deletions in protein C.
The capsid protein, C, of tick-borne encephalitis virus has recently been found to tolerate deletions up to a length of 16 amino acid residues that partially removed the central hydrophobic domain, a sequence element conserved among flaviviruses which may be crucial for virion assembly. In this study, mutants with deletion lengths of 19, 21, 27, or 30 residues, removing more or all of this hydr...
متن کاملTwo distinct size classes of immature and mature subviral particles from tick-borne encephalitis virus.
Flaviviruses assemble in the endoplasmic reticulum by a mechanism that appears to be driven by lateral interactions between heterodimers of the envelope glycoproteins E and prM. Immature intracellular virus particles are then transported through the secretory pathway and converted to their mature form by cleavage of the prM protein by the cellular protease furin. Earlier studies showed that whe...
متن کاملDistinguishing West Nile virus infection using a recombinant envelope protein with mutations in the conserved fusion-loop
BACKGROUND West Nile Virus (WNV) is an emerging mosquito-transmitted flavivirus that continues to spread and cause disease throughout several parts of the world, including Europe and the Americas. Specific diagnosis of WNV infections using current serological testing is complicated by the high degree of cross-reactivity between antibodies against other clinically relevant flaviviruses, includin...
متن کاملIsolation and characterization of tick-borne encephalitis virus from Ixodes persulcatus in Mongolia in 2012.
Tick-borne encephalitis virus (TBEV) is a zoonotic virus belonging to the genus Flavivirus, in the family Flaviviridae. The virus, which is endemic in Europe and northern parts of Asia, causes severe encephalitis. Tick-borne encephalitis (TBE) has been reported in Mongolia since the 1980s, but details about the biological characteristics of the endemic virus are lacking. In this study, 680 tick...
متن کامل